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Fig. 6. Mislocalized grk mRNA at the anterior is dynamic. FRAP experiments were performed on K10; grk^*GFP and grk^*GFP; sqd¹ egg chambers. Images of grk^*GFP (A), K10; grk^*GFP (B,D) and grk^*GFP; sqd¹ (C) egg chambers during mid-to-late stage 9. (B-D) Mislocalized grk^*GFP forms an anterior ring at the edge of the oocyte. A defined area of fluorescent grk mRNA at the dorsal (arrowhead) and ventral-anterior (arrow) sides was bleached during FRAP experiments. (E) Previously we noted that grk^*GFP localized at the dorsal-anterior cap during mid-to-late stage 9 had very reduced recovery (25% recovery) during FRAP experiments. By contrast, grk^*GFP found both at the dorsal and ventral-anterior sides of K10 and sqd¹ mutants was more dynamic (45%). (F) Bar graph representing the half-time of recovery (t_1/2) for grk^*GFP during mid-to-late stage 9 from controls and mutant lines. Recovery rates are much faster in the mutants, similar to the younger stages in the control line (Fig. 4). A minimum of five FRAP experiments were taken for each data point. Error bars represent standard deviations.

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