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Fig. 4. LPA-induced adhesion movement is FAK dependent. (A) Analysis of adhesion movement in response to 2 µM LPA. Control or FAK-siRNA-treated Rat2 cells stably expressing paxillin-GFP were plated on fibronectin-coated glass T dishes i medium containing 10% serum. The following day, cells were serum-starved for 12 hours and stimulated with 2 µM LPA. Adhesion movement was analyzed using TIRF microscopy as described in Fig. 3. Cells were filmed for 27 minutes before the addition of LPA and 27 minutes after the addition of LPA. (B) Quantification of dynamic adhesions was assessed as described in Materials and Methods (arrows indicate representative adhesions). The experiment was performed three times, six to eight cells were analyzed per experimental condition.
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