|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 26 February 2008
doi: 10.1242/jcs.023077
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
Institute of Plant Sciences and Zurich-Basel Plant Science Center, Swiss Federal Institute of Technology, ETH Centre, CH-8092 Zurich, Switzerland
* Author for correspondence (e-mail: koehlerc{at}ethz.ch)
Accepted 8 January 2008
 |
Summary |
|---|
 Top Summary IntroductionResultsDiscussionMaterials and MethodsReferences |
|---|
Key words: Arabidopsis, Epigenetics, FERTILIZATION INDEPENDENT SEED genes, DNA methylation, Polycomb group proteins
|
Introduction |
|---|
|
TopSummaryIntroductionResultsDiscussionMaterials and MethodsReferences |
|---|
). In mammals, imprinted genes regulate placental development and fetal growth, and several human diseases are linked to mutations in imprinted genes (Reik, 2007). Mechanisms to distinguish maternal and paternal alleles have been extensively investigated in mammals. Most mammalian imprinted genes are clustered in the genome and are regulated by differentially methylated imprinting-control regions (ICRs) (Edwards and Ferguson-Smith, 2007). Different DNA methylation marks are applied during germ-line formation by de novo methyltransferases and are maintained in somatic tissues by maintenance methyltransferases. ICRs are also marked by different histone modifications and can either act as insulators preventing promoter-enhancer interactions or give rise to the formation of non-coding RNAs that attract chromatin-modifying complexes (Delaval and Feil, 2004). Some imprinted genes are regulated by Polycomb group (PcG) complexes that methylate histones and establish repressive chromatin domains (Delaval and Feil, 2004).
In flowering plants, imprinting has only been detected in the endosperm, a terminal tissue that develops after fertilization of the central cell. In dicot species like Arabidopsis, the endosperm nourishes the embryo during its growth phase and is almost completely consumed during embryo development (Berger, 2003). Thus far, only four imprinted genes have been identified in Arabidopsis. Three of them [MEDEA (MEA), FWA and FERTILIZATION INDEPENDENT SEED 2 (FIS2)] are maternally expressed and paternally silenced (Vielle-Calzada et al., 1999; Kinoshita et al., 2004; Jullien et al., 2006b). The same paternally imprinted expression pattern applies to all imprinted genes that have been identified in maize (Scott and Spielman, 2006). Paternal imprinting of MEA requires activity of the evolutionary conserved FIS-PcG complex, with MEA itself being a subunit of this complex. The FIS complex mediates trimethylation of histone H3 at the lysine residue at position 27 (H3K27me3) of the paternal MEA allele causing its repression (Baroux et al., 2006; Gehring et al., 2006; Jullien et al., 2006a). Activation of the maternal MEA allele in the female gametophyte requires the 5-methylcytosine excising activity of the DNA glycosylase DEMETER (DME). DME acts antagonistically to the maintenance methyltransferase MET1 that methylates MEA in the promoter and 3' regions of the gene (Xiao et al., 2003). DME activity is also necessary to activate expression of the paternally imprinted genes FWA and FIS2. Because MEA, FWA and FIS2 are only demethylated in the terminally differentiating endosperm, they remain heritably methylated throughout the life cycle of the plant (Xiao et al., 2003; Kinoshita et al., 2004; Jullien et al., 2006b).
The promoter region of FWA and the 3' region of MEA contain tandem-repeat sequences that recruit de novo DNA methylation by the RNA-dependent DNA silencing pathway (Chan et al., 2006b). Whereas FWA tandem repeats are necessary to establish FWA silencing (Chan et al., 2006b), silencing of the paternal MEA allele is likely to be independent of the repeat sequences (Spillane et al., 2004).
The type I MADS-box gene PHERES1 (PHE1) is the only plant gene known to be paternally expressed and maternally silenced. Maternal PHE1 silencing is caused by the repressing activity of the FIS complex (Köhler et al., 2005). The FIS complex is active in the female gametophyte and in the endosperm, prevents precocious PHE1 expression before fertilization and restricts PHE1 expression to the chalazal domain of the endosperm after fertilization. The FIS complex is directly associated with the PHE1 locus and FIS repressive activity is correlated with H3K27me3 modification at PHE1 (Köhler et al., 2003; Makarevich et al., 2006). Upregulation of PHE1 in mea mutants is in part responsible for the mea mutant phenotype that can be alleviated by reducing PHE1 expression (Köhler et al., 2003).
In this study, we asked whether FIS-mediated repression is sufficient for PHE1 imprinting or whether additional mechanisms are involved to repress the maternal PHE1 allele. Our data clearly show that imprinting is not a direct consequence of FIS-mediated repression but necessitates the presence of additional elements. We identified elements within the PHE1 3' region that are necessary for PHE1 imprinting and predict a model that explains how the FIS complex – together with the identified region – confers stable silencing of the maternal PHE1 allele.
|
Results |
|---|
|
TopSummaryIntroductionResultsDiscussionMaterials and MethodsReferences |
|---|
; Edwards and Ferguson-Smith, 2007). Therefore, we tested whether imprinting of PHE1 is also regulated by DNA methylation. Mutations in the maintenance MEHYLTRANSFERASE 1 (MET1) gene cause a drastic reduction of symmetric CG methylation (Kankel et al., 2003), whereas mutations in the CHROMOMETHYLASE 3 (CMT3) gene and the de novo methyltransferases DRM1 and DRM2 affect CNG and asymmetric methylation, respectively (Cao and Jacobsen, 2002). We pollinated met1, cmt3 and drm1/drm2 double mutants with pollen of C24 wild-type plants and analyzed allele-specific expression of PHE1. As shown in Fig. 1A and supplementary material Fig. S1A and Fig. S3, in none of the mutants a reactivation of the maternal PHE1 allele was detectable and the paternal PHE1 allele remained the predominantly expressed allele. In conclusion, DNA methylation is not responsible for repression of the maternal PHE1 allele.
|
). Previously we observed strongly reduced PHE1 expression levels in the decrease in DNA methylation 1 (ddm1) mutant, which is impaired in the maintenance of DNA methylation (Köhler et al., 2003). This prompted us to test whether DNA methylation is required for expression of the paternal PHE1 allele. We did not detect substantial PHE1 expression in pollen (data not shown); however, fertilization of wild-type plants with hypomethylated pollen of met1 mutants caused much lower PHE1 transcript levels in developing seeds than fertilization with wild-type pollen (Fig. 2A), indicating reduced activity of the paternal PHE1 allele. This suggests that DNA methylation is important to maintain high levels of paternal PHE1 expression, but is not necessary to repress the maternal PHE1 allele. When we tested allele-specific PHE1 expression in seeds developing after pollination with met1 pollen, we clearly detected reduced expression of the paternal PHE1 allele (Fig. 2B), which supports our conclusion that DNA methylation is required for paternal PHE1 expression. However, we also detected expression of the maternal PHE1 allele in seeds that inherited a hypomethylated paternal PHE1 allele (Fig. 2B and supplementary material Fig. S1B), suggesting that relieve of repression of the maternal PHE1 allele depends on additional not-yet-known factors. Because DNA methylation appears to be important for PHE1 regulation, we asked the question which regions of the PHE1 locus contain DNA methylation. Using bisulfite sequencing we did not detect any DNA methylation within the promoter and coding regions of PHE1. Therefore, we extended our analysis to regions downstream of the PHE1 locus. We performed Southern blot analysis using the restriction enzyme ClaI, which is blocked by symmetric methylation at its target recognition site. Using this assay we detected a methylated site 2538 bps downstream of the PHE1 stop codon (Fig. 3A). No annotated genes are located in this region. Using Southern blot analysis and bisulfite sequencing we detected no substantial methylation at this site in the met1 mutant (Fig. 3A,B). Thus, reduced methylation at this site in met1 correlates with reduced PHE1 expression. Interestingly, this methylated ClaI site is located in close proximity to a tandem triple repeat, each repeat being 54 bps long. Bisulfite sequencing of the region surrounding the ClaI site revealed twelve consecutive methylated cysteine residues in a symmetric CG context and two methylated CNG sites (supplementary material Fig. S3). We performed tissue-specific analysis of DNA methylation in pollen, flowers and leaves. Consistent with our findings that in met1 mutants the paternal allele is less expressed, we detected high levels of DNA methylation in pollen at all sites tested (Fig. 3B). However, we also detected substantial DNA methylation in sporophytic tissues where only weak PHE1 expression is detectable, suggesting that DNA methylation alone is not sufficient to confer active PHE1 expression.
|
|
Disruption of the PHE1 3' regions causes activation of the maternal PHE1 allele
Our results suggest that DNA methylation in the identified PHE1 3' region is important for PHE1 imprinting. In order to test this hypothesis we addressed the question whether disruption of the PHE1 3' region disrupts PHE1 imprinting. We identified a T-DNA mutant containing a 4 kb T-DNA insertion 441 bps downstream of the PHE1 stop codon (referred to as phe1-3) (Fig. 4A). Using this line, we could test whether an insertion within the 3' region disrupts PHE1 imprinting. We tested allele-specific expression of PHE1 in phe1-3 by performing reciprocal crosses of phe1-3 with C24 plants. Whereas expression of the paternal PHE1 allele was not affected in phe1-3 (Fig. 4B, upper panel and supplementary material Fig. S1C), we observed a drastic effect on the expression of the maternal allele when phe1-3 was used as the maternal parent (Fig. 4B lower panel and supplementary material Fig. S1C). In phe1-3 mutants, PHE1 was not maternally silenced but, in contrast to wild-type, which has only a weak expression of the maternal PHE1 allele, this allele was strongly expressed (Fig. 4B, lower panel). Surprisingly, we detected a decrease in the expression of the paternal PHE1 allele when phe1-3 was used as the maternal parent (cross phe1-3 x C24; Fig. 4B and supplementary material Fig. S1C). One possible explanation for this phenomenon might be the triploid nature of the endosperm consisting of two maternal versus one paternal genome copies. Therefore, if the two maternal PHE1 alleles become reactivated, they might outcompete expression of the single paternal PHE1 allele. We also considered the possibility that strong expression of the maternally derived PHE1 allele in phe1-3 is caused by de-repression of PHE1 in maternal sporophytic tissues of phe1-3 plants. Therefore, we tested expression of PHE1 in phe1-3 leaves. However, as shown in Fig. 4C, PHE1 remains as weakly expressed in phe1-3 leaves as in wild-type leaves. We further considered the possibility that transcripts derived from the T-DNA can influence PHE1 expression. To test this possibility, we performed northern blot analysis using probes flanking the insertion site. However, we did not obtain any expression signal with either of the probes, but detected a clear expression signal for the NEOMYCIN PHOSPHOTRANSFERASE II (NPTII) selection gene that is located within the T-DNA (Fig. 4D). Finally, we tested PHE1 imprinting in two additional transgenic lines containing a 4 kb T-DNA or a 6.6 kb Ds transposon insertion 1022 bps or 1168 bps after the PHE1 stop codon, respectively. The T-DNA in the insertion line (referred to as phe1-4) is inserted in the antisense orientation compared with the T-DNA of the phe1-3 allele, whereas insertion of the Ds element (referred to as phe1-5) is in the same orientation as phe1-3 (Fig. 4A). Consistent with the results obtained for the phe1-3 allele, we observed reactivation of the maternal PHE1 allele and a reduction of paternal PHE1 expression (Fig. 4E and supplementary material Fig. S1C), whereas we did not observe an effect on expression of the paternal PHE1 allele in those mutants (data not shown). Taken together, these results clearly demonstrate that the 3' region of PHE1 contains elements necessary for repression of the maternal PHE1 allele.
|
) (Fig. 6B).
|
|
Our results suggest that DNA methylation of the paternal PHE1 allele is necessary for paternal PHE1 expression (Fig. 2). To substantiate these findings we transformed the PHE13000::GUS_3' construct into a drm1/drm2 mutant background. DRM1 and DRM2 are necessary for de novo cytosine methylation in all known sequence contexts and are guided to their templates by small interfering (si) RNAs (Chan et al., 2004). It has previously been demonstrated that transgene sequences when transformed into a drm1/drm2 mutant background remain unmethylated owing to lack of de novo methyltransferase activity (Chan et al., 2004). Indeed, we did not detect significant levels of DNA methylation of the transgene in the drm1/drm2 mutant background (Fig. 5). Using this transformation assay allowed to directly address the question whether DNA methylation of the PHE13000::GUS_3' transgene affects PHE1 expression and avoids secondary effects caused by global DNA demethylation. We tested allele-specific PHE1 expression by crossing wild-type plants with pollen from drm1/drm2; PHE13000::GUS_3' transgenic lines. In none of the three tested independent transgenic lines did we detect expression of the paternally derived PHE13000::GUS_3' construct (Fig. 6C). Thus, DNA methylation of the 3' region of the paternal PHE1 allele is necessary for PHE1 expression. Taken together, our data clearly demonstrate that the PHE1 downstream region contains important sequence elements necessary for imprinting of PHE1.
|
Discussion |
|---|
|
TopSummaryIntroductionResultsDiscussionMaterials and MethodsReferences |
|---|
). De novo methylation of repeats depends on the de novo methyltransferase DRM2 that is guided by siRNAs using the RNA-directed DNA methylation pathway (Chan et al., 2006b). Whereas DRM2-mediated de novo methylation occurs in all sequence contexts, DRM2-mediated maintenance methylation is restricted to CNG and asymmetric cytosine residues (Cao and Jacobsen, 2002). The identified direct repeats in the PHE1 3' region are preferentially methylated on symmetric CG residues, indicating that methylation of PHE1 repeats depends on MET1 maintenance methyltransferase activity. Thus, methylation of PHE1 repeats is likely to occur through similar mechanisms as methylation of repeats in the promoter of the imprinted FWA gene. Methylation of endogenous FWA repeats depends on MET1 activity, whereas methylation of transgenic FWA repeats depends on DRM2 activity (Kinoshita et al., 2004; Chan et al., 2006b). However, in contrast to the role of DNA methylation for silencing of the paternal FWA allele, we found DNA methylation being necessary for expression of the paternal PHE1 allele. This conclusion is supported by two findings: (1) endogenous paternal PHE1 expression is reduced in a met1 mutant and, (2) PHE13000::GUS_3' is not paternally expressed when transformed into the de novo methyltransferase mutant drm1/drm2 that is deficient in de novo methylation of repeated transgene sequences during plant transformation (Chan et al., 2004; Chan et al., 2006b). As PHE1-repeat sequences are also methylated in tissues where PHE1 is not substantially expressed, we conclude that DNA methylation is necessary but not sufficient to determine PHE1 activity. It is possible that additional activating signals present only during seed development are necessary for PHE1 expression. Surprisingly, we detected expression of the maternal PHE1 alleles in seeds inheriting a hypomethylated paternal PHE1 allele. One possible explanation for this finding could involve recruitment of the FIS complex to the demethylated paternal PHE1 allele. If the number of FIS complexes is limited, additional FIS target genes could cause a reactivation of silenced maternal PHE1 alleles.
Is there a difference in DNA methylation between maternal and paternal alleles? We failed to solve this question for technical reasons, as it requires DNA isolation of either female gametophytes or pure endosperm tissues. However, given the presence of active DNA-demethylating enzymes in Arabidopsis, with DME having an assigned function of demethylation in the central cell, it is possible that PHE1 becomes demethylated in the central cell of the female gametophyte. DME is necessary for MEA and FIS2 expression, and lack of DME function causes seeds to abort with a fis-like phenotype. As MEA is necessary for repression of the maternal PHE1 allele, a direct function of DME for PHE1 regulation cannot be unequivocally addressed.
|
; Kinoshita et al., 2004; Jullien et al., 2006b). Our model provides an explanation for the seemingly contradictory findings that demethylation of the paternal PHE1 allele causes paternal PHE1 repression, whereas insertions of transgene sequences do not impair PHE1 expression. We propose that the demethylated paternal PHE1 allele becomes repressed, either by formation of repressive chromatin loops or the action of silencing elements. By contrast, insertions of long transgene sequences in the 3' PHE1 region will negatively interfere with loop formation or inhibit the long range action of silencing elements, resulting in active PHE1 expression. We failed to detect antisense transcripts generated within the 3' PHE1 region in wild-type and met1-mutant plants (data not shown), suggesting that long antisense RNAs are not involved in PHE1 imprinting.
Similar to the situation for PHE1, several imprinted genes in mammals depend on DNA demethylation for silencing, whereas DNA methylation is necessary for expression (Sleutels and Barlow, 2002). Similar to the model proposed by Sleutels and Barlow (Sleutels and Barlow, 2002), we assume that imprinting of PHE1 evolved in two steps. First, PHE1 expression became silenced by insertion of a repetitive sequence into a regulatory region located in the 3' region of the gene. In a second step this repetitive sequence became methylated, thereby causing restoration of PHE1 expression. Owing to a female-gamete-specific demethylation activity, the maternal PHE1 allele is silenced, whereas the paternal allele is methylated and active. This model makes two predictions, (1) removal of the insertion mutation that causes the silencing effect should reactivate the silenced allele and, (2) loss of DNA methylation should result in silencing. Indeed, both predictions are supported by the results presented in this study. We could demonstrate that removal of the PHE1 3' region causes de-repression of the maternal PHE1 allele and loss of DNA methylation causes silencing of the paternal PHE1 allele. The effect of DNA methylation to suppress the silencing effect of transposons has long been appreciated in plants (Martienssen, 1998) and was probably used as a mechanism to achieve active PHE1 expression in male gametes.
|
Materials and Methods |
|---|
|
TopSummaryIntroductionResultsDiscussionMaterials and MethodsReferences |
|---|
). The met1 mutant used in this study corresponds to the met1-3 allele (Col accession) described by Saze et al. (Saze et al., 2003). For all experiments with met1 mutants, only first-generation homozygous plants were used. The drm1/drm2 double mutant (Wassilewskija accession) was obtained from the Nottingham Arabidopsis Stock Centre (CS6366). The cmt3-11 allele corresponds to SALK_148381 (Col accession) (Chan et al., 2006a). The mutant alleles phe1-3, phe1-4 and phe1-5 correspond to SALK_023774 (Col accession), SALK_010202 (Col accession) and GT_5_108060 (Ler accession), respectively. The PHE13000::GUS line (Ler accession) have been described by Köhler et al. (Köhler et al., 2003). Plants were grown in a greenhouse at 70 % humidity and daily cycles of 16 hours light at 21°C and 8 hours darkness at 18°C. Developed gynoecia were emasculated and hand pollinated one day after emasculation. For RNA expression analysis, three gynoecia or siliques were harvested at the indicated time points.
Plasmid constructs and generation of transgenic plants
To generate the PHE13000::GUS_3' construct, a 3000 bp sequence upstream of the PHE1 translational start and the PHE1-coding region were amplified by PCR, introducing EcoRI and XmaI restriction sites. The fragment was ligated into pCAMBIA1381Xc, creating an in-frame fusion with the GUS gene. Using EcoRI and BstEII sites, a fragment containing the PHE1 promoter, PHE1 coding region and the GUS gene was removed and introduced together with a fragment containing 3540 bps of PHE1 3' region flanked by BstEII and PstI sites into pCAMBIA 3300. The PHE13000::GUS_3' construct was introduced into homozygous drm1/drm2 plants and the corresponding Wassilewskija accession.
RNA extraction and qPCR analysis
RNA extraction and generation of cDNAs were performed as described previously (Köhler et al., 2005). Quantitative PCR was done on an ABI Prism 7700 Sequence Detection System (Applied Biosystems) using SYBR Green PCR master mix (Applied Biosystems) according to the supplier's recommendations. The mean value of three replicates was normalized using ACTIN11 as control. The primers used in this study are specified in the supplementary material (Table S1).
Allele-specific expression analysis
Allele-specific PHE1 expression was analyzed using the assay described by (Köhler et al., 2005). The amplified products were digested with HphI and analyzed on a 2.5% agarose gel. All primers are specified in the supplementary material (Table S1).
GUS expression analysis
Staining of seeds to detect GUS activity was done as described previously (Köhler et al., 2003).
Northern and Southern blot analysis
Northern and Southern blot analyses were performed as described previously (Köhler et al., 2003). Primers used to generate probes are specified in supplementary material Table S1.
Bisulfite sequencing
Bisulfite sequencing was performed following the protocol of (Jacobsen et al., 2000). Used primers are specified in supplementary material Table S1. We sequenced six clones covering the regions of 1672-2144 bp (primers Fwd1 and Rev1) and 2099-2470 bp (primers Fwd2 and Rev2) and ten clones of the region 2425-2783 bp (primers Fwd3 and Rev3). We sequenced 12 clones covering the region 2099 bp-2470 bp (primers Fwd2 and Rev2) of transgenic lines containing the PHE13000::GUS_3' construct in wild-type and drm1/drm2-mutant background.
|
Acknowledgments |
|---|
|
Footnotes |
|---|
|
References |
|---|
|
TopSummaryIntroductionResultsDiscussionMaterials and MethodsReferences |
|---|
Baroux, C., Gagliardini, V., Page, D. R. and Grossniklaus, U. (2006). Dynamic regulatory interactions of Polycomb group genes, MEDEA autoregulation is required for imprinted gene expression in Arabidopsis. Genes Dev. 20, 1081-1086.
Berger, F. (2003). Endosperm, the crossroad of seed development. Curr. Opin. Plant Biol. 6, 42-50.[CrossRef][Medline]
Cao, X. and Jacobsen, S. E. (2002). Locus-specific control of asymmetric and CpNpG methylation by the DRM and CMT3 methyltransferase genes. Proc. Natl. Acad. Sci. USA 99 Suppl. 4, 16491-16498.
Chan, S. W., Zilberman, D., Xie, Z., Johansen, L. K., Carrington, J. C. and Jacobsen, S. E. (2004). RNA silencing genes control de novo DNA methylation. Science 303, 1336.
Chan, S. W., Henderson, I. R., Zhang, X., Shah, G., Chien, J. S. and Jacobsen, S. E. (2006a). RNAi, DRD1, and histone methylation actively target developmentally important non-CG DNA methylation in Arabidopsis. PLoS Genet. 2, e83.[CrossRef][Medline]
Chan, S. W., Zhang, X., Bernatavichute, Y. V. and Jacobsen, S. E. (2006b). Two-step recruitment of RNA-directed DNA methylation to tandem repeats. PLoS Biol. 4, e363.[CrossRef][Medline]
Delaval, K. and Feil, R. (2004). Epigenetic regulation of mammalian genomic imprinting. Curr. Opin. Genet. Dev. 14, 188-195.[CrossRef][Medline]
Edwards, C. A. and Ferguson-Smith, A. C. (2007). Mechanisms regulating imprinted genes in clusters. Curr. Opin. Cell Biol. 19, 281-289.[CrossRef][Medline]
Feil, R. and Berger, F. (2007). Convergent evolution of genomic imprinting in plants and mammals. Trends Genet. 23, 192-199.[CrossRef][Medline]
Gehring, M., Huh, J. H., Hsieh, T. F., Penterman, J., Choi, Y., Harada, J. J., Goldberg, R. B. and Fischer, R. L. (2006). DEMETER DNA glycosylase establishes MEDEA Polycomb gene self-imprinting by allele-specific demethylation. Cell 124, 495-506.[CrossRef][Medline]
Grossniklaus, U., Vielle-Calzada, J. P., Hoeppner, M. A. and Gagliano, W. B. (1998). Maternal control of embryogenesis by MEDEA a Polycomb group gene in Arabidopsis. Science 280, 446-450.
Jacobsen, S. E., Sakai, H., Finnegan, E. J., Cao, X. and Meyerowitz, E. M. (2000). Ectopic hypermethylation of flower-specific genes in Arabidopsis. Curr. Biol. 10, 179-186.[CrossRef][Medline]
Jullien, P. E., Katz, A., Oliva, M., Ohad, N. and Berger, F. (2006a). Polycomb group complexes self-regulate imprinting of the Polycomb group gene MEDEA in Arabidopsis. Curr. Biol. 16, 486-492.[CrossRef][Medline]
Jullien, P. E., Kinoshita, T., Ohad, N. and Berger, F. (2006b). Maintenance of DNA methylation during the Arabidopsis life cycle is essential for parental imprinting. Plant Cell 18, 1360-1372.
Kankel, M. W., Ramsey, D. E., Stokes, T. L., Flowers, S. K., Haag, J. R., Jeddeloh, J. A., Riddle, N. C., Verbsky, M. L. and Richards, E. J. (2003). Arabidopsis MET1 cytosine methyltransferase mutants. Genetics 163, 1109-1122.
Kinoshita, T., Miura, A., Choi, Y., Kinoshita, Y., Cao, X., Jacobsen, S. E., Fischer, R. L. and Kakutani, T. (2004). One-way control of FWA imprinting in Arabidopsis endosperm by DNA methylation. Science 303, 521-523.
Köhler, C. and Makarevich, G. (2006). Epigenetic mechanisms governing seed development in plants. EMBO Rep. 7, 1223-1227.[CrossRef][Medline]
Köhler, C., Hennig, L., Spillane, C., Pien, S., Gruissem, W. and Grossniklaus, U. (2003). The Polycomb-group protein MEDEA regulates seed development by controlling expression of the MADS-box gene PHERES1. Genes Dev. 17, 1540-1553.
Köhler, C., Page, D. R., Gagliardini, V. and Grossniklaus, U. (2005). The Arabidopsis thaliana MEDEA Polycomb group protein controls expression of PHERES1 by parental imprinting. Nat. Genet. 37, 28-30.[CrossRef][Medline]
Makarevich, G., Leroy, O., Akinci, U., Schubert, D., Clarenz, O., Goodrich, J., Grossniklaus, U. and Köhler, C. (2006). Different Polycomb group complexes regulate common target genes in Arabidopsis. EMBO Rep. 7, 947-952.[CrossRef][Medline]
Martienssen, R. (1998). Transposons, DNA methylation and gene control. Trends Genet. 14, 263-264.[CrossRef][Medline]
Reik, W. (2007). Stability and flexibility of epigenetic gene regulation in mammalian development. Nature 447, 425-432.[CrossRef][Medline]
Saze, H., Scheid, O. M. and Paszkowski, J. (2003). Maintenance of CpG methylation is essential for epigenetic inheritance during plant gametogenesis. Nat. Genet. 34, 65-69.[CrossRef][Medline]
Scott, R. J. and Spielman, M. (2006). Deeper into the maize, new insights into genomic imprinting in plants. BioEssays 28, 1167-1171.[CrossRef][Medline]
Sleutels, F. and Barlow, D. P. (2002). The origins of genomic imprinting in mammals. Adv. Genet. 46, 119-163.[Medline]
Spillane, C., Baroux, C., Escobar-Restrepo, J. M., Page, D. R., Laoueille, S. and Grossniklaus, U. (2004). Transposons and tandem repeats are not involved in the control of genomic imprinting at the MEDEA locus in Arabidopsis. Cold Spring Harb. Symp. Quant. Biol. 69, 465-475.[CrossRef][Medline]
Vielle-Calzada, J. P., Thomas, J., Spillane, C., Coluccio, A., Hoeppner, M. A. and Grossniklaus, U. (1999). Maintenance of genomic imprinting at the Arabidopsis MEDEA locus requires zygotic DDM1 activity. Genes Dev. 13, 2971-2982.
Xiao, W., Gehring, M., Choi, Y., Margossian, L., Pu, H., Harada, J. J., Goldberg, R. B., Pennell, R. I. and Fischer, R. L. (2003). Imprinting of the MEA Polycomb gene is controlled by antagonism between MET1 methyltransferase and DME glycosylase. Dev. Cell 5, 891-901.[CrossRef][Medline]
Zhang, X., Yazaki, J., Sundaresan, A., Cokus, S., Chan, S. W., Chen, H., Henderson, I. R., Shinn, P., Pellegrini, M., Jacobsen, S. E. et al. (2006). Genome-wide high-resolution mapping and functional analysis of DNA methylation in Arabidopsis. Cell 126, 1189-1201.[CrossRef][Medline]
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  J. N. F. Gerald, P. S. Hui, and F. Berger Polycomb group-dependent imprinting of the actin regulator AtFH5 regulates morphogenesis in Arabidopsis thaliana Development, October 15, 2009; 136(20): 3399 - 3404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. B. R. Villar, A. Erilova, G. Makarevich, R. Trosch, and C. Kohler Control of PHERES1 Imprinting in Arabidopsis by Direct Tandem Repeats Mol Plant, July 1, 2009; 2(4): 654 - 660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ishikawa and T. Kinoshita Epigenetic Programming: The Challenge to Species Hybridization Mol Plant, July 1, 2009; 2(4): 589 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gehring, K. L. Bubb, and S. Henikoff Extensive Demethylation of Repetitive Elements During Seed Development Underlies Gene Imprinting Science, June 12, 2009; 324(5933): 1447 - 1451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-F. Hsieh, C. A. Ibarra, P. Silva, A. Zemach, L. Eshed-Williams, R. L. Fischer, and D. Zilberman Genome-Wide Demethylation of Arabidopsis Endosperm Science, June 12, 2009; 324(5933): 1451 - 1454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dominguez and F. Berger Chromatin and the cell cycle meet in Madrid Development, November 1, 2008; 135(21): 3475 - 3480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tiwari, R. Schulz, Y. Ikeda, L. Dytham, J. Bravo, L. Mathers, M. Spielman, P. Guzman, R. J. Oakey, T. Kinoshita, et al. MATERNALLY EXPRESSED PAB C-TERMINAL, a Novel Imprinted Gene in Arabidopsis, Encodes the Conserved C-Terminal Domain of Polyadenylate Binding Proteins PLANT CELL, September 1, 2008; 20(9): 2387 - 2398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Makarevich, C. B. R. Villar, A. Erilova, and C. Kohler Mechanism of PHERES1 imprinting in Arabidopsis Development, April 1, 2008; 135(7): e1 - e1. [Full Text]
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
