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Figure 2


Fig. 2. TGFβ1-ALK1 and TGFβ1-ALK5 signaling pathways in L6E9 cells. Cells were serum starved for 24 hours before TGFβ1 treatments (30 minutes for p-Smads, 1 hour for Id1 and 24 hours for PAI1). (A) Cells were treated with the ALK5 inhibitor SB431542 (SB, 5 µM) 1 hour before treatment with TGFβ1. Whole-cell extracts were analyzed by western blot with anti-pSmad1, anti-pSmad2 and collagen I. Total protein extracts from control (C) or TGFβ1-treated (T) myoblasts (1 hour) were analyzed by western blot with anti-Id1 (B) and anti-PAI1 (D). L6E9 were transiently transfected with (Bre)2-Luc (C), and (CAGA)12-Luc (E) reporters; cells were incubated or not with TGFβ1 for 24 hours, before measuring the luciferase activity. Results are represented as fold induction of the TGFβ1 treated over the untreated counterparts. The histogram represents the mean of three independent experiments. *P<0.05, Student's t-test.





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