spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Effect of L- and S-endoglin on collagen I and CTGF expression. (A,B) Mock, L- and S-endoglin cells were treated (T) or not (C) with TGFβ1 for 24 hours in serum-free medium. Total protein extracts were analyzed by western blot using a specific antibody for collagen I (A) or CTGF (B). Measures of densitometry of each band were performed and relative values are represented. Collagen I and CTGF histogram represents the mean of three different extracts. (C) L6E9 cells were transfected with a vector expressing either caALK1 or caALK5, and collagen expression analyzed by western blot. (D) Mock, L-Endo and S-Endo-transfected cells were treated with the ALK5 inhibitor SB431542 (SB, 5 µM) 1 hour before treatment with TGFβ1. Total protein extracts from control (C) or TGFβ1-treated (T) myoblasts were analyzed by western blot with anti-collagen I and anti-tubulin antibodies. A representative blot from three independent experiments is shown. The blot of the S-endoglin samples is under-exposed in order to visualize the differences caused by the SB431542 treatment.





Right arrow Return to article