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Figure 1


Fig. 1. Gene expression in primary fetal liver cells following MACS. (a,b) Gene expression with two-color immunostaining in EpCAM-positive cells to demonstrate coexpression of Alb and CK-19 or vimentin (arrows). DAPI nuclear counterstain, blue color. Magnification: x200. (c) Gene expression by RT-PCR amplification in EpCAM-positive or -negative cell fractions, including hepatobiliary markers (Alb, CK-19), liver transcription factors (hepatic nuclear factors, HNFs) (lanes 1, 2), growth factors and receptors (lanes 3, 4) and cell-signaling molecules Wnt receptors, frizzled (FZ) and Notch (lanes 5, 6).





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