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Fig. 1. Inhibiting expression of nuclear lamin proteins using RNAi. RNAi was used to inhibit lamin protein expression in HeLa cells and analysis was performed on individual cells. (A,B) RNAi was performed by transient expression and transfection monitored by co-transfection of a vector expressing YFP-tubulin (green). At selected times after transfection (48 hours in this example) cells were fixed and immunolabelling of the residual lamina performed (red). Histograms (C) show averaged data (fluorescent intensities expressed as arbitrary units; n>75) from three equivalent experiments in which the average expression of lamin B1 and A/C was reduced (KD) to 20.7 and 19.8% of untransfected cells (Control) in the same samples. As negative controls, cells were transfected with empty vector (not shown) or appropriate scrambled RNAi (C). Scale bars: 10 µm.
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