spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Dissociating epithelial junctions regulate SRF via MAL. (A) Effects of MAL constructs or the F-actin stabilising mutant actin G15S. 0.4 µg MAL (f.l.), 2.5 µg MAL {Delta}N{Delta}B1, 2.5 µg MAL {Delta}N{Delta}C, 0.4 µg MAL {Delta}N (scheme) or 2.5 µg actin G15S were co-transfected and Ca2+ exchange was carried out as in Fig. 3A. Luciferase activity is normalised to pRL-TK, with error bars indicating s.e.m. (n=3). (B) Dissociation of the actin-MAL complex upon Ca2+ withdrawal. MDCK cells were electroporated with 10 µg Flag-actin and 10 µg MAL-HA. 36 hours later, the medium was exchanged to low Ca2+ for the times indicated and cell lysates were immunoprecipitated with anti Flag. Proteins were visualised using antibodies against Flag and HA-tags. Latrunculin B (3 µM) or cytochalasin D (10 µM) was added 30 minutes before medium exchange. Relative association was calculated from densitometric analysis. Mock, medium exchange with 1.8 mM Ca2+; –Ca2+, medium exchange with 0.02 mM Ca2+.





Right arrow Return to article