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Figure 6


Fig. 6. TGF-β1 induces EMT transcriptional responses in MTEC in a JNK1-dependent manner. (A) TGF-β1-induced gene expression changes obtained via microarray analysis are blunted in JNK1 MTEC compared with wild-type cells. Data plot shows the fold change (2n) in gene expression induced by TGF-β1 compared to sham controls (TGF-β1-treated, sham controls) for wild-type MTEC (x-axis) and JNK1–/– MTEC (y-axis). The dashed line depicts the normal distribution expected if the gene expression changes were similar in wild-type and JNK1–/– MTEC. The solid line depicts the mean gene expression changes observed, and indicate a blunted response to TGF-β1 in JNK1–/– MTEC. Horizontal and vertical gray dotted lines represent the twofold change cutoffs. (B) TGF-β1-dependent modulation of mesenchymal and epithelial gene expression in MTEC depends on JNK-1. MTEC were treated with 5 ng/ml TGF-β1 for the indicated times and RNA evaluated by Taqman analysis. Data are represented as fold change in expression compared to sham controls (n=2, representative of five independent experiments). (C) TGF-β1 enhanced expression of known EMT regulatory transcription factors in a JNK1-dependent manner as assessed by Taqman analysis. * P<0.05, ANOVA versus wild-type MTEC. CCSP, clara cell secretory protein; Col1a1, collagen type 1a1; Fn1, fibronectin 1; HMGA 2, high mobility group AT-hook 2; Jag-1, jagged-1; PAI-1, plasminogen activator inhibitor 1.





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