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Figure 7


Fig. 7. Evaluation of TGF-β1 pathway activation in wild-type and JNK1–/–-deficient MTEC. (A) Assessment of nuclear accumulation of phospho-Smad2 (P-Smad2), Smad2, P-Smad3, Smad3, Smad4 and P-Jun in wild-type or JNK1–/– MTEC after exposure to 5 ng/ml TGF-β1 for 120 or 240 minutes. (B) Assessment of binding of nuclear proteins to the consensus SBE via electrophoretic mobility shift analysis in wild-type or JNK1–/– MTEC after exposure to 5 ng/ml TGF-β1 for 120 or 240 minutes. Different lanes shown represent results from independent samples.





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