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Figure 1


Fig. 1. TERT overexpression does not maintain telomere length under hyperoxia. (A) Telomere restriction fragment length in MRC5-TERT cells grown for the indicated times (in days) under normoxia (left) or under 40% hyperoxia (right). Population doublings are indicated (PD). M indicates the positions of a {lambda}HINDIII size marker. (B) Telomere shortening rates per PD in MRC5 (black bars) and MRC5-TERT (white bars) under normoxia (left) and hyperoxia (right). Data are mean±s.e.m. from four experiments measured in quadruplicate. The asterisk indicates a significant difference between parental and TERT-overexpressing cells with P<0.05 (ANOVA). (C) Metaphase spreads from MRC5 (left) and MRC5-TERT (right) cells grown under hyperoxia for the indicated times (in days) hybridised with a telomeric PNA probe (red). Chromosomes are stained with DAPI (blue). (D) Frequency distributions of telomere fluorescence signal intensities in MRC5-TERT (top) and MRC5 (bottom) cells grown under hyperoxia for the indicated times. Red bars indicate the median telomere length. (E) Frequencies of chromosomal end-to-end fusions per metaphase in MRC5 (black) and MRC5-TERT (white, broken lines) under hyperoxia. Data are mean±s.e.m. from at least 25 metaphases per condition.





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