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Figure 3


Fig. 3. Localization of AgRho1 proteins. N-terminal fusions of yellow fluorescent protein (YFP) to AgRho1a and cyan fluorescent protein (CFP) to AgRho1b localizing at the tip (A) and at the septum (B). The slides were incubated for 1 hour at the given temperature prior to microscopy. The overlay uses green for YFP-AgRho1a and red for CFP-AgRho1b. Bar, 10 µm (A); 5 µm (B). (C) Colocalization of GFP-AgRho1b with 1,3-β-glucan biosynthesis at the tip. Mycelia that were grown on microscopy slides were incubated with 1 mg/ml Aniline Blue in AFM for visualization of 1,3-β-glucan biosynthesis for 5-10 minutes prior to microscopy. Aniline Blue is shown in red and GFP-AgRho1b in green on the overlay image. Bar, 10 µm.





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