JCS024927 Supplementary Material

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• Supplemental Figure S1 -

  Fig. S1. PI(4)P localizes to Golgi and acroblast membranes. (A,E) Phase-contrast and (B-D,F-H) corresponding fluorescence micrographs showing spermatocytes (A-D) and spermatids (E-H) co-expressing the Golgi marker Fws-GFP (green) and RFP-PH-FAPP (red), which binds PI(4)P. (D,H) Merged images showing colocalization of Fws-GFP and RFP-PH-FAPP (yellow). (D) PI(4)P co-localizes with Fws-GFP to the Golgi in spermatocytes (A-D) and to the acroblast, a Golgi-derived organelle, in spermatids (E-H). Scale bar, 20 µm.