JCS026492 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. COS-7 cell co-expressing GFP-Kif5c as shown in Fig. 1A. 50 frames. Frame capture rate: 1 frame every 5 seconds. (Saved at 10 frames per second.)

• Movie 2 -

  Movie 2. COS-7 cell co-expressing GFP-Kif5c as shown in Fig. 1B. Frame rate: 1 frame every 5 seconds. (Total: 40 frames, saved at 10 frames per second.)

• Movie 3 -

  Movie 3. Neuronal cell co-expressing GFP-Kif5c as shown in Fig. 1C. Frame rate: 1 frame every 0.5 seconds. (Total: 100 frames, saved at 10 frames per second.)

• Movie 4 -

  Movie 4. COS-7 cell co-expressing GFP-Kif5c as shown in Fig. 1D. Frame rate: 1 frame every 0.5 seconds. (Total: 100 frames, saved at 10 frames per second.)

• Movie 5
• Movie 6 -

  Movies 5 and 6. Two cropped regions from a COS-7 cell co-expressing GFP-Kif5c and mCherry-tubulin, frame capture rate, 1 frame every 2 seconds, as shown in Fig. 2. The regions show a single GFP-Kif5c fluorescent spot moving along a single mCherry-tubulin labelled microtubule. The epifluorescence images were taken using a deconvolution microscope (Deltavision), and a small Z-stack (3) was collected at each time point. The resulting images were deconvolved and the middle stack of the images is shown. (Saved at 10 frames per second.)

• Movie 7 -

  Movie 7. COS-7 cell expressing GFP-Kif5c show an accumulation of GFP-Kif5c at the periphery. After photobleaching, this accumulation gradually recovers. Images were recorded using a Zeiss confocal microscope at a frame rate of 1 frame every six seconds (as shown in Fig. 4B). The bleaching is indicated by the filled grey circle (frames 4-6) followed by the subsequent frames at 1 frame every six seconds. (Total: 60 frames, saved at 10 frames per second.)

• Movie 8 -

  Movie 8. A cropped region from a COS-7 cell, expressing GFP-Kif5c, after 40 minutes of nocodazole treatment. Images taken every 5 seconds. GFP-Kif5c has accumulated on a small number of microtubules and moves towards the end of these microtubules. (60 frames, saved at 20 frames per second.)

• Movie 9 -

  Movie 9. COS-7 cell co-expressing GFP-Kif5c and mCherry-tubulin, imaged every 5 minutes, after nocodazole addition at time=0. A Z-stack of 5 images was taken at each time point, and the image has been deconvolved. A maximum intensity image from the stack is shown (as shown in Fig. 4C). (Total: 12 frames, saved at 10 frames per second.)