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Figure 1


Fig. 1. mis4 mutants are sensitive to HDAC inhibition and show synthetic lethality with HDAC mutants. (A) Spot tests of effects of TSA on the growth of a kinetochore mutant mis6-302 and HDAC mutants clr6-1, nmt1-clr6HA, {Delta}clr3, {Delta}hos2 and wild type (WT). These strains were grown at 26°C and spotted at serial cell concentrations on YPD plates lacking or containing TSA (12.5 µg/ml), followed by incubation at the indicated temperatures. (B) Wild type, mis4-242, rad21-K1, psc3-407 and eso1-H17 mutants were spotted on YPD plates lacking or containing TSA (25.0 µg/ml). (C) mis4-242 mutants were cultured in YPD medium containing 12.5 µg/ml TSA at 26°C (upper panel) or in YPD medium at 36°C (lower panel) for 6 hours, then fixed with glutaraldehyde and stained with DAPI. The cells showing unequal chromosome missegregation phenotypes are indicated by arrowheads, and the cells in which chromosomes were condensed because of mitotic arrest due to spindle checkpoint activation by arrows. Scale bar: 10 µm. (D) The indicated strains were grown exponentially and then spotted on YPD plates.





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