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Fig. 1. Homogeneous Xi size and appearance in confluent female human fibroblasts (WI-38 cells). (A-C) DAPI staining, blue; histone H3-3mK27 immunostaining (mouse antibody) marking Xi, red. (A) 85% of cells show a recognizable, condensed Barr body in non-deconvolved single optical sections (95% if 3D data analyzed). (B,C) The remaining cells either show multiple DAPI-intense bodies of similar size to the Barr body (B) or no distinct DAPI bright body (C). (D) Histogram showing Xi area (µm²), based on H3-3mK27 immunostaining, for confluent versus log-phase cells. (E,F) H3-3mK27 immunostaining of Xi in log-phase cells frequently shows a fibrillar substructure protruding from the Xi core. Original image, top framed area; corresponding deconvolved optical section, lower inset. Arrows indicate the position of the Xi. Scale bar: 2 µm.

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