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Figure 4


Fig. 4. Xi and chromocenters have a different ultrastructure. (A) Fluorescent images of a mouse embryonic fibroblast nucleus counterstained by DAPI (blue) show the Xi (arrows) identified by mouse anti H3-3mK27 antibody (pink) with weaker DAPI intensity than observed in chromocenters (arrowheads) but still noticeably above the background DAPI intensity level. (B,C) Electron micrographs of 200-nm sections of two nuclei demonstrate looser chromatin packing of the Xi (arrows) compared with that of chromocenters (some marked by arrowheads). (C) Nanogold staining of histone H3-3mK27 (rabbit antibody) shows the comparison of the labeled Xi facultative heterochromatin (arrow) and heterochromatin of the adjacent chromocenter (arrowhead). Nu, nucleolus.





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