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Figure 6


Fig. 6. Serial sections (200 nm) reveal the Xi–nuclear-envelope association even when the Xi appears interior in equatorial sections (confluent WI-38 cells). (A-C) Three sections of the same immunostained (H3-3mK27) nucleus with nanogold-labeled heterochromatin of the Xi (arrows). (A) The equatorial section 4 shows the Xi and adjacent nucleolus (Nu) in the nuclear interior. (B,C) Top-two grazing sections of the above nucleus (section 2, section 1) show the attachment of the Xi and adjacent nucleolus to the overlying nuclear periphery. (D) An example of the apparent intranuclear Xi (arrow) attached to the deep nuclear envelope invagination (Inv).





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