spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. PAK is necessary for Rac1-induced destabilization of E-cadherin complexes from junctions. (a, left panels) Rac1Q61L was co-expressed with kinase-dead PAK1K299R or with the auto-inhibitory domain Myc-PAKAID. Seven hours post-injection, cells were fixed and immunostained for epitope tags and {alpha}-catenin. (Right panels) As controls, Rac1Q61L, PAKK299R or Myc-tagged PAKAID were microinjected alone and stained as above. Arrow indicates absence of {alpha}-catenin. Arrowheads show the presence of {alpha}-catenin at cell-cell contacts. Scale bars, 20 µm. (b) Effects of co-expression of constitutively active Rac1 and PAK mutants were quantified by measuring the ratio of the length in cadherin staining to the length of the cell-cell contact (corner to corner of neighbouring expressing cells). Controls were arbitrarily set as 100% (length in cadherin staining = length of the cell-cell contact). *P<0.05; **P<0.01. (c) Keratinocytes express PAK1 mRNA, but not PAK2 or PAK3 mRNAs. Keratinocyte and monocyte RNAs were subjected to RT-PCR using primers specific for members of the class I PAK family.





Right arrow Return to article