|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 4. GAS5-specific siRNAs regulate the growth of CEM-C7 cells and delay growth arrest. CEM-C7 T-leukemic cells were transfected with specific siRNAs targeting GAS5 or with negative control siRNA [(–)siRNA] and cultured at 37°C. (A) After 72 hours the expression of endogenous GAS5 in the siRNA transfected CEM-C7 cells was determined by real-time RT-PCR (mean ± s.e.m. from six separate experiments). (B) Viable cell numbers were determined by vital-dye staining (mean ± s.e.m. from six independent experiments). (C) Caspase activation, as a marker of apoptosis, was determined 72 hours after transfection (mean ± s.e.m. from six independent experiments). (D) DNA content of CEM-C7 transfected with negative-control siRNA or specific GAS5 siRNAs was monitored at different time points by PI staining and flow cytometry (see Table 1 for quantification). *P<0.01 compared with (–)siRNA.
|
