spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. AR binds to importin-{alpha} through its hinge region in vitro and in mammalian cell extracts. (A) AR constructs coding for the GST-tagged N-terminal transactivation domain (TAD), GST-tagged DNA-binding domain (DBD), GST-tagged hinge (H) domain, GST-tagged ligand-binding domain (LBD) or GST-tagged LBD and H domain together (H-LBD), were overexpressed and immobilised on glutathione-S-sepharose (GSH) resin. wtAR, wild-type AR. (B) After incubation with purified importin-{alpha}, beads were washed, proteins eluted and separated, and probed for importin-{alpha} by western analysis (1:2000). Equal loading of GST-AR domains was verified by Coomassie staining of an identical gel, with GST-AR domains marked by an asterisk. (C) AR was immuno-precipitated from COS-7 cells and probed for bound importin-{alpha} by western blotting. Immunoprecipitation was carried out with and without androgen (10 nM R1881).





Right arrow Return to article