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Figure 4


Fig. 4. The AR hinge region has an intrinsic nuclear import function in live cells. COS-7 cells were transfected with GFP-AR constructs corresponding to wtAR, or disease-related mutations (R617P, C619Y, R629W, R629Q, K630T) or mutations predicted to alter nuclear import from the crystal structure (M624D, K630A, K632A, K633A, KKK630/2/3AAA). Cells were treated with 10 nM non-hyrolysible androgen (R1881, + androgen) or vehicle (ethanol,–androgen) for 30 minutes or 48 hours, fixed with methanol and mounted in Vectorshield. Cells were imaged by confocal microscopy. GFP is shown in green. Images of the two top rows show the cytoplasmic location of wtAR and the different AR mutants in the absence of androgen. The middle and bottom rows show the nuclear translocation of the different AR constructs in the presence of androgen after 30 minutes and 48 hours, respectively. Scale bars, 10 µM.





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