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Fig. 1. Minigenes of human lamin A. (A,B) Constructs used for tetop-LA^wt (A) and tetop-LA^G608G (B) transgenic mice. Both constructs contain a minigene, including the coding region of lamin A (exons 1-10, intron 11 and exon 12), and differ only in nucleotide 1824, where tetop-LA^wt+ carries the wild-type lamin A sequence, and the tetop-LA^G608G+ carries the most common HGPS mutation, 1824C>T. tetop, Tet-operon; IRES, internal ribosomal entry site; eGFP, coding region for enhanced green fluorescent protein. (C) The tet-off system used in this study. Bi-transgenic animals, tetop-LA^G608G+; K5tTA⁺, express human LA, LAdel50 (progerin) and green fluorescent protein (GFP). In the presence of doxycycline (DOX) the transcription is turned off.

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