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Figure 6


Fig. 6. Expression of dominant-negative (DN) Pak2 or Pak4 blocks EC lumen formation in 3D collagen matrices. ECs infected with GFP, Pak2 or Pak4 DN mutant adenoviruses (Ad) were suspended within 3D collagen matrices for 24 hours. (A) Graph showing the mean number of EC lumens per HPF±s.d. (n=3). *, P<0.03 compared with GFP control. (B) Representative fields of ECs infected with the indicated adenoviruses. Arrows indicate EC lumen formation. Bar, 50 µm.





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