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Figure 3


Fig. 3. F-actin regulation is impaired by the loss of FrmA, and F-actin and FrmA colocalise. (A) Confocal images of wild-type (left) and frmA– (middle) cells fixed and stained with TRITC-phalloidin (red) and DAPI (blue) to highlight the actin cytoskeleton and the nucleus, respectively. Cross-sectional images were captured and the maximum projections shown. F-actin-rich patches were located at the cortex of frmA– cells and in particular at the cell-substrate boundary. The frmA– image closest to the cell-substrate boundary, is shown on the right with white arrows highlighting patches and yellow lines indicating the cross section being shown above and beside the layer. (B) TIRF images showing LimE{Delta}coil:GFP localisation in wild-type (left panels) and frmA– cells (right panels). (C) Confocal images of frmA–/FrmAHA cells, fixed and stained with TRITC-phalloidin and an anti-HA antibody conjugated to FITC to highlight the actin cytoskeleton and localisation of FrmAHA, respectively. Images closest to the cell-substrate boundary are shown. Specific areas (rectangles 1, 2 and 3) were further magnified and shown immediately below with arrows highlighting F-actin patches and FrmAHA colocalisation.





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