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Figure 4


Fig. 4. Starvation-induced development and directed cell migration is impaired in frmA– cells. (A) Transmitted light images of cells taken at 0 and 10 hours of starvation. (B) The speed of non-starved, randomly migrating cells and starved cells undergoing cell migration towards a micropipette containing 2 µM cAMP was determined and expressed as a percentage of the wild type. 15 cells were tracked (using Image J software) from each strain on three separate occasions and the average speed ± s.e.m. is shown. (C) RT-PCR of total RNA from non-starved and 6-hour-starved cells was used to determine the expression of cAR1, g{alpha}2 and FrmA. Expression of eF1{alpha} was used as a template loading control. (D) Consecutive and merged TIRF (green) and transmitted light (red) images of randomly moving non-starved cells. (E) Consecutive and merged TIRF (green) and transmitted light (red) images of 6-hour-starved cells migrating towards a micropipette containing 2 µM cAMP (white asterisks).





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