spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Selective inhibition of BCL10- and CARMA3-induced NF-{kappa}B activation by A20. (A,B) HEK293 cells were transiently cotransfected with an expression vector encoding the indicated polypeptides, together with NF-{kappa}B–luciferase and β-galactosidase reporter vectors. The total amount of transfected plasmid DNA was maintained constant by adding empty vector. 16 hours after transfection, cell lysates were prepared and luciferase activity was measured. The data shown represent the relative luciferase activity normalized against β-galactosidase activity and are representative of six independent experiments performed in triplicate. Bottom panels: a fraction of the transfection reaction mixture was separated by SDS-PAGE, blotted onto nitrocellulose membrane and analyzed by immunoblotting to monitor protein expression.





Right arrow Return to article