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Figure 4


Fig. 4. A20 inhibits formation of molecular complexes following activation of PKC. HEK293 cells were transfected with an empty expression plasmid or plasmid encoding A20. 24 hours later, cells were treated with PMA (40 ng/ml) plus ionomycin (1 µM) for 40 minutes. Cell extracts were immunoprecipitated with the indicated immunoprecipitating antibody (IP) and assayed for coprecipitating proteins by immunoblot experiments probed with the indicated antibody (WB).





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