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Figure 6


Fig. 6. The effect of siRNA-mediated knockdown of SHP2 on phosphorylation of p66Shc and JNK. (A,B) Whole-cell lysates of E5-expressing HDFs transfected with a control siRNA (250 pmoles) or a SHP2-specific siRNA (250 or 500 pmoles) were subjected to immunoblotting for SHP2, Ser36 phosphorylated p66Shc (PS36-p66Shc) or phosphorylated JNK (pJNK) as indicated. In A, the PS36-p66Shc blot was reprobed for total p66Shc and the SHP2 blot was reprobed for β-tubulin. In B, the pJNK blot was reprobed for total JNK and SHP2. JNK isoforms 1 and 2 are indicated.





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