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Figure 1


Fig. 1. E/nestin:dVenus fluorescence intensity is correlated with Nes mRNA expression. (A) Structure of the E/nestin:dVenus transgene. (B) Emission spectra of Venus (green) and EGFP (red). (C,D) On an identical section from an E14 E/nestin:dVenus-EGFP double transgenic embryo, no dVenus-expressing cells showed immunoreactivity against the anti-β-tubulin III antibody, although some EGFP-expressing cells did (arrowheads). VZ, ventricular zone; IZ, intermediate zone. (E) Representative FACS profile of cerebral-wall-derived cells from an E14.5 E/nestin:dVenus transgenic embryo. (F) Quantitative data showing the percentage of E/nestin:dVenus negative (F1) and positive (F2) cells immunostained with nestin, β-tubulin III and GFP antibodies. (G) To compare the E/nestin:dVenus fluorescence intensity with the Nes mRNA expression level, real-time quantitative PCR was performed. dVenus-positive cells were subdivided into two fractions based on their fluorescence intensity: high (F4) and low (F3). The Nes mRNA expression level of these two fractions (F4 and F3), as well as of the dVenus-negative cells (F1), showed a positive correlation with the fluorescence intensity. Data are means ± s.e.m. Scale bars: 500 µm (C), 25 µm (D).





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