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Fig. 2. Cell-cycle-dependent expression of Nes. (A) Schematic diagram of the timing used to administer thymidine analogues to label cells in each cell-cycle phase (see Materials and Methods for details). (B-D) IdU (red) was injected for 0.5 hour (B), 3 hours (C) and 14.5 hours (D) to label cells in S, G2-M and G1 phases, respectively. BrdU (purple) was injected 30 minutes before sacrifice to label cells in S phase. dVenus (green) was expressed in many of the cells in G1 and S phase (arrowheads), but cells in the G2-M phase were mostly dVenus negative (arrows). (E) Ratio of IdU-positive cells that expressed dVenus to the total number of IdU-positive cells, counted for each cell-cycle phase. Cells in G1 and S phase expressed the highest levels of dVenus, whereas its expression levels were much lower during G2-M phase. Scale bar: 25 µm (B-D). Data represent the mean ± s.e.m.
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