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Figure 1


Fig. 1. Increased expression of C/EBPβ in mouse glial primary cultures after treatment with different stimuli. (A) Western blot showing an increase in C/EBPβ expression in microglial cells treated with LPS and KA and in astrocytes treated with LPS, staurosporine, KA or glutamate for 24 hours. Only a small induction was observed in astrocyte cultures after treatment with KA or glutamate. (B,C) Primary astroglial (B) and microglial (C) cultures prepared from C/EBPβ+/+ were treated as in A, and C/EBPβ expression was evaluated by immunofluorescence analysis and confocal microscopy using a specific anti-C/EBPβ antibody (clone A16), as described in the Materials and Methods. Cultures were also stained with anti-CD11b (clone M1/70.15) or anti-GFAP antibodies to detect microglia or astrocytes, respectively. (D,E) To confirm the specificity of the anti-C/EBPβ antibody, primary microglial (D) and astroglial (E) cultures from C/EBPβ–/– mice were treated with LPS for 24 hours, and C/EBPβ expression was evaluated by immunofluorescence analysis and confocal microscopy. Representative results from three experiments are shown. Scale bar, 10 µm. Nuclei were counterstained by DAPI (blue). B, basal; LPS, lipopolysaccharide; St, staurosporine; KA, kainic acid; Glu, glutamate.





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