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Fig. 3. Induction of C/EBPβ expression after KA injection in the adult mouse brain. (A) Immunohistochemical analyses of coronal brain sections prepared from C/EBPβ^+/+ mice 24 and 72 hours after KA injection. C/EBPβ protein levels were substantially enhanced in the stratum radiatum (SR) and in the granular layer (GL) of the dentate gyrus of the hippocampus of C/EBPβ^+/+ mice 24 hours and 72 hours post injection, compared with vehicle-injected controls. Images are representative for experiments from five animals. Scale bar, 25 µm. (B) Double immunofluorescent labeling and confocal microscopic analyses of C/EBPβ after KA injection in C/EBPβ^+/+ mice. Double-labeling studies were performed with an antibody recognizing C/EBPβ (green) and different reagents to detect astrocytes (anti-GFAP), microglia (tomato lectin), and neurons (Neurotrace) (red labeling). Complete colocalization (yellow) was found in neurons, although 24 hours and 72 hours after KA injection some astrocytes and microglia were found that express C/EBPβ (arrows). Scale bar, 10 µm. (C) Immunohistochemical analyses of coronal brain sections prepared from C/EBPβ^–/– mice 24 hours and 72 hours after KA injection. As expected, C/EBPβ^–/– mice did not present any C/EBPβ staining. Images are representative for experiments from five animals. Scale bar, 25 µm.

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