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Fig. 7. Deficiency of C/EBPβ protects neurons from excitotoxic brain damage. (A,B) C/EBPβ^+/+ and C/EBPβ^–/– adult mice were injected with KA and sacrificed 24 hours and 72 hours post-injection. Coronal sections (30 µm) were stained with (A) Fluoro-Jade B and (B) anti-NeuN antibody. C/EBPβ^–/– mice exhibited low levels of neuronal degeneration (as detected by Fluoro-Jade B staining) and a diminished loss of neurons in the CA1 and CA3 regions (as shown by NeuN staining) when compared with littermate controls. Scale bars, 10 µm (A) and 25 µm (B). (C) Quantification of degenerating neurons analyzed in the CA1 and CA3 areas of the hippocampus. Values represent the mean ± s.e. from five different animals and two independent sections per animal. (D) The extent of neuronal damage in the CA1 and CA3 areas of the hippocampus was quantified as described in Materials and Methods. Data were normalized against the mean values obtained from vehicle-injected mice. Values represent the mean ± s.e. from five different animals and two independent sections per animal. ^*P0.05, ^**P0.01, ^***P0.001, C/EBPβ^–/– mice versus C/EBPβ^+/+ mice at each time point.

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