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Figure 5


Fig. 5. The PECAM-1 {Delta}15 isoform fails to mediate full protection against apoptosis induced by overexpression of Bax and by treatment with etoposide. (A) HEK293T cells were transiently transfected with plasmids encoding the indicated proteins. 24 hours after transfection, the cells were lysed and their caspase-3 activity was determined, as described in Materials and Methods. As reported previously, WT PECAM-1 protected against apoptosis induced by overexpression of Bax, but neither the ITIM-less nor the {Delta}15 form of PECAM-1 provided cytoprotection against overexpression of Bax. (B) REN mesothelioma cells expressing approximately equivalent amounts of WT PECAM-1 or {Delta}15 PECAM-1 were generated. After 48 hours, caspase-3 activity was determined. Cells expressing {Delta}15 PECAM-1 provided far less cytoprotection than that afforded by WT PECAM-1 (C). The values shown are the mean±s.d. The results are representative of at least two independent experiments performed in duplicate or triplicate; *P<=0.0002, **P<=0.05, derived using unpaired Student's t test.





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