spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. SNX9 binds to dynamin 2 and N-WASP through its SH3 domain and colocalizes on tubules. (A) Pull-down assay from HEK 293T cells with purified GST fusion of SNX9, SNX9-SH3, SNX9-{Delta}SH3 or GST-alone. The pull-downs were analyzed by Coomassie Blue staining. Arrow indicates major interacting protein predicted to be dynamin 2. Immunoblotting using anti-dynamin 2 or anti-N-WASP antibody was then performed. (B) COS-7 cells were cotransfected with Flag-tagged SNX9 or SNX9-{Delta}SH3 and GFP-tagged N-WASP or GFP-tagged dynamin 2, and immunoprecipitation (IP) was carried out with anti-Flag antibody, followed by immunoblotting (IB) with anti-Flag or anti-GFP antibody. TCL, total cell lysates. (C,D) Dual-color simultaneous time-lapse imaging of SNX9 or SNX9-{Delta}SH3 with dynamin 2 or N-WASP. GFP- or mRFP-tagged SNX9 or SNX9-{Delta}SH3 was cotransfected with mRFP-tagged dynamin 2 or GFP-tagged N-WASP, respectively. Live dual-color imaging was performed 24 hours after transfection. High-magnification views are of the regions enclosed in rectangles. Scale bars: 10 µm (low magnification), 2 µm (insets).





Right arrow Return to article