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Fig. 7. Perturbation of actin dynamics enhances tubule formation by SNX9. (A) COS-7 cells were transfected with low levels of GFP-SNX9, which does not induce membrane tubulation (left). After addition of latrunculin B (1 µM), tubular invaginations can be observed within a few seconds (right). Insets are high-magnification views and below are time-lapse images of the regions enclosed in rectangles. (B) Cells expressing low levels of GFP-SNX9 were imaged before and after the addition of jasplakinolide (2 µM). Scale bar: 30 µm; 2 µm for insets and time-lapse images. (C) COS-7 cells expressing GFP-tagged SNX9 was stained with 15 µM FM5-95 (C) for 10 minutes on ice and treated with latrunculin B. SNX9-induced tubules (green) and FM5-95-stained plasma membranes (red) were mostly colocalized. High-magnification views are of the regions enclosed in rectangles. Scale bars: 30 µm (low magnification), 5 µm (insets).
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