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Figure 2


Fig. 2. Influence of irofulven on the dynamics of Pol II LS and induction of post-translational modifications. (A) Mammalian cells expressing GFP-Pol II were subjected to FLIP analysis under different experimental conditions: untreated cells, cells treated with 100 µM DRB, cells treated with 5 µg/ml actinomycin D, cells treated with 5 µg/ml irofulven and cells treated with 5 µg/ml irofulven and 100 µM DRB together. All curves represent an average of at least 12 independent measurements. (B) HeLa cells were incubated with 1 µg/ml irofulven for the indicated times. Total (upper panel), H14-reactive (second panel) and H5-reactive (third panel) levels of Pol II LS were assessed by immunoblotting, with actin (last panel) serving as an internal control for equal loading. Band intensities were measured, and the indicated IIO ratios were calculated as [(IIO/total Pol II LS)x100]. (C) Overexposed Pol II LS immunoblot.





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