JCS017210 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Adobe PDF

• Supplemental Figure S2 -

  Adobe PDF

• Supplemental Figure S3 -

  Adobe PDF

• Supplemental Figure S4 -

  Adobe PDF

• Supplemental Table S1 -

  Adobe PDF

• Supplemental Table S2 -

  Adobe PDF

• Movie 1 -

  Movie 1. Time-lapse movie of cell shown in Fig. 2A. Control GFP-tubulin cell with no RNAi treatment. Images were acquired every 10 seconds.

• Movie 2 -

  Movie 2. Time-lapse movie of cell shown in Fig. 2B. Mob4-RNAi-treated cell with monoastral bipolar spindle. Images were acquired every 10 seconds.

• Movie 3 -

  Movie 3. Time-lapse movie of cell shown in Fig. 2C. Mob4-RNAi-treated cell with two centrosomes and bipolar spindle. Images were acquired every 15 seconds.

• Movie 4 -

  Movie 4. Time-lapse movie of Mob4-RNAi-treated GFP-tubulin cell with two active centrosomes. Images were acquired every 10 seconds. Only the centrosome on the left participates in spindle formation, while the centrosome on the right does not. A monoastral bipolar spindle begins to form as a result.

• Movie 5 -

  Movie 5. Time-lapse movie of Mob4-GFP tubulin cell shown in Fig. 7. Images were acquired every 10 seconds. With the exception of a slight decrease in Mob4-GFP fluorescence at the kinetochore pair on the mono-oriented chromosome just before it is aligned on the metaphase plate, Mob4-GFP is present at both spindle poles and kinetochores and weakly throughout the spindle at various stages of mitosis.