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Figure 2


Fig. 2. Drosophila Mob4 (Dmob4) has a minor roll in the maintenance of centrosome number and functions in spindle pole organization in the absence of centrosomes. (A) Bars indicate the percentage of mitotic cells with 0, 1, 2, 3, 4 or >=5 centrosomes (Dgrip84-stained foci that nucleate astral microtubules) in untreated cells (tan; n=401) or cells treated with RNAi to Mob4 (green; n=408) or SAK polo-like kinase (blue; n=400). (Error bars indicate s.d. of mean value of two independent experiments.) Depletion of Mob4 increased the proportion of single-centrosome cells relative to untreated cells but did not generate a significant population of acentrosomal cells, as did RNAi of SAK. (B) RNAi of SAK led to loss of centrosomes and astral microtubules from spindle poles, and co-depletion of Mob4 with SAK noticeably exacerbated spindle fiber unfocusing at acentrosomal poles (right). ({alpha}-tubulin, red; Dgrip84, green; DNA, blue; bar, 5 µm.) (C) Histogram showing the distribution of spindle pole focal breadth (yellow line; the distance between the minus ends of the outermost K fibers) at acentrosomal poles in SAK-depleted cells (blue) and in cells depleted of both Mob4 and SAK by double RNAi (red). The mean focal breadth in Mob4-SAK co-depleted cells was 5.0±2.4 µm (n=246), whereas that in cells depleted of SAK alone was 1.9±1.4 µm (n=261; P<0.0001). (Error bars indicate s.d. of mean value of two independent experiments.)





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