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Figure 5


Fig. 5. Drosophila Mob4 fused to GFP (Dmob4-GFP) accumulates at mitotic spindle poles and kinetochores. (A) In interphase cells, Mob4-GFP (green in A-D) localizes primarily to the nucleus. ({alpha}-tubulin, red; DNA, blue for A and B.) (B) Mob4-GFP is diffuse throughout the cytoplasm in mitotic cells but accumulates both at spindle poles and at punctate spots immediately adjacent to condensed chromosomes (arrows in Mob4-GFP panel). (C) Mob4-GFP colocalizes with {gamma}-tubulin staining (red) at the centrosome. (DNA, blue.) (D) Mob4-GFP foci adjacent to condensed chromosomes (arrows in Mob4-GFP panel) coincide with immunostaining for the fly CENP-A homolog CID (red). The spindle is oriented diagonally from upper left to lower right. The inset shows a magnified image of the chromosome pair at the upper left in the main panels, taken in an alternative Z section. Bar, 5 µm (2 µm for inset in D). (One CID pair does not appear to have a matching GFP-Mob4 pair because the fluorescent intensity of Rhodamine-stained CID was substantially larger than the GFP signal and bled onto focal planes in which the GFP signal was not visible.)





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