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Figure 6


Fig. 6. Time-lapse imaging of mitosis in cells expressing Drosophila Mob4 fused to GFP (Dmob4-GFP). Pairs of GFP foci indicating kinetochores are visible on both mono-oriented chromosomes (left two panels) and on chromosomes aligned at the metaphase plate. In some cases (such as the cell shown), kinetochore fluorescence of mono-oriented chromosomes was stronger before alignment at the metaphase plate than after, but otherwise GFP fluorescence at spindle poles and kinetochores is present throughout mitosis. The time in minutes and seconds from beginning of image acquisition is indicated at the lower right of each panel. Bar, 5 µm. The full time-lapse movie is shown in supplementary material Movie 5.





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