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Fig. 7. Ultrastructural analysis of
ypt1/SLY1-20 cells. Wild-type, wild-type expressing SLY1-20 and
ypt1/SLY1-20 strains were grown to early to mid-log phase and half of the culture was shifted to 37°C for 1 hour. The cultures were processed for ultrastructural analysis. Thin sections were stained with lead citrate and uranyl acetate. (A-D) Cells grown at 23°C; (E-H) cells shifted for 1 hour to 37°C. (A,E) wild-type; (B,F) wild-type expressing SLY1-20; (C,D,G,H)
ypt1/SLY1-20. White arrowheads point to individual Golgi cisternae. The black arrowhead in G points to an accumulation of vesicles [an enlargement (x2) is shown in the inset]. The black arrowhead in H is directed towards strange membraneous profiles. An enlargement (x2) is shown in the inset. N, nucleus; V, vacuole. Bar (in A), 500 nm.