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Figure 8


Fig. 8. Ypt1p interacts directly with Ufe1p-GST. The transmembrane domain of the SNAREs was replaced by GST. Equal amounts of GST-tagged SNAREs were immobilized on GSH-agarose and incubated with purified His6-Ypt1p. His6-Ypt1p bound to the SNAREs was detected by immunoblotting (lanes 1-10). The amount of immobilized SNARE proteins is shown in lanes 11-20.





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