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Figure 3


Fig. 3. wasp3D3-035 and wipD30 single mutants stop fusion during fusion-pore formation, whereas, in Arp3schwächling mutants, a fusion pore is formed. (A-D) Ultrathin sections of stage-14 embryos show myoblasts in the process of fusion (boxed area). (A'-D') Higher-magnification views of the boxed areas. (A,A') Wild type. (A') A forming fusion pore (arrows) and vesiculating membranes (arrowheads). (B) Myoblast fusing to a precursor cell in a wasp3D3-035 embryo. (B') The higher-magnification view shows that membranes are vesiculating. (C) In Arpschwächling mutant embryos, a trinucleated precursor cell is surrounded by several myoblasts. (C') A fusion pore has formed between the precursor cell and the FCM, but the FCM fails to integrate into the muscle. (D) Fusing myoblasts in a wipD30 (vrp1) embryo. (D') Vesiculating membranes. N, cell nucleus. Magnification: (A-D) 3600x; (A',D') 12,000x; (B',C') 21,000x.





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