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Figure 7


Fig. 7. Duf is localized correctly in wasp3D3-035 and Arp3schwächling mutants, suggesting that assembly of the FuRMAS occurs. (A-D) Anti-Duf staining on stage-15/16 embryos. (A) In a wild-type embryo, Duf is expressed on the site of the FC/growing myotube as a ring (frontal view, arrowhead). The asterisks mark FCMs attaching to a growing myotube (lateral view). (B) Three FCMs attach to a growing myotube (asterisks, lateral view) in an Arp3schwächling embryo. Duf is present on the site of the FC/growing myotube. (C) In a wasp3D3-035 embryo, the asterisks once again highlight FCMs (lateral view) that attach to growing myotubes expressing Duf. (D) In Arp3schwächling wasp3D3-035 double-mutant embryos, Duf is also expressed and concentrated at the site of cell-cell contact. Three FCMs can be seen to attach to a Duf-expressing cell (asterisks). Scale bars: 10 µm.





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