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Fig. 3. Rearward traction stresses in myosin-II-null cells are identical to those in wild-type cells. (A) Simultaneous recording of the fluorescence of GFP-ABD120k (left) and traction stresses (right) of a migrating myosin-II-null cell transformed with a GFP-ABD120k construct under confocal microscopy. Stress spots (a'-c') appeared at locations where F-actin accumulated (a-c) in the anterior and middle regions of the cell. The typical sequential stress map shown was generated from three migrating cells randomly selected from 13 cells. The time-courses are indicated for each picture of traction stresses. The yellow arrows at 0 seconds indicate the direction of cell migration. (B) Simultaneous recording of the fluorescence of GFP-ABD120k (left) and IRM imaging (right) of a migrating myosin-II-null cell transformed with a GFP-ABD120k construct under confocal microscopy. Dark spots surrounded by white rings (d'-f') appear at the locations where F-actin had accumulated beforehand (d-f). Bars, 4 µm. (C) Average distance between the centers of each F-actin accumulation and corresponding stress spot in wild-type and myosin-II-null cells. (D) Average interval between the time at which the fluorescence of GFP-ABD120k at the center of each F-actin accumulation reached its maximum value and the time at which the stress value at the corresponding stress spot reached its maximum. (E) Average maximum values of rearward traction stresses for each stress spot in wild-type and myosin-II-null cells. (F) Average stress spot duration times for wild-type and myosin-II-null cells. There was no significant difference between wild-type cells and myosin-II-null cells in the maximum magnitude or duration of stress at each stress spot.
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