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Fig. 8. The motor activity of myosin II is required for the generation of traction forces by retracting pseudopodia. (A) Dynamics of E476K myosin II filaments (upper images) and traction stresses (lower images) under a pseudopod of a migrating myosin-II-null cell expressing GFP-E476K myosin II. The stress map was made from three migrating cells randomly selected from six cells. E476K myosin II filaments began to accumulate in the pseudopod during elongation of the pseudopod (0-24 seconds), similar to wild-type myosin II. After elongation, traction stress slightly increased only under the tip of the pseudopod (arrows). Bar, 1.5 µm. (B) Time-courses of pseudopod area (blue) and fluorescence intensity of GFP-myosin-II (red). (C) Time-courses of fluorescence intensity of GFP-myosin-II (red) and average traction stress in the pseudopod (blue). The increase in pseudopod area was followed by the accumulation of E476K myosin II in the pseudopod, as was the case for normal myosin II (Fig. 7B). However, the traction stress increased only at the tip of the retracting pseudopod.
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