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Figure 1


Fig. 1. UBPY and AMSH localize to the central spindle and midbody during cytokinesis. (A) Classification of the four stages (S1~S4) of cytokinesis used in this study. HeLa cells were double-stained for microtubules (red) and DNAs (blue) with anti-tubulin antibody and TO-PRO-3, respectively. See text for details. (B-B'') HeLa cells at stage 4 of cytokinesis were triple-stained with anti-UBPY antibody (B), anti-tubulin antibody (B', red) and TO-PRO-3 (B', blue). (C-C'') HeLa cells were transfected with FLAG-tagged UBPY, and triple-stained with anti-FLAG antibody (C), anti-tubulin antibody (C', red) and TO-PRO-3 (C', blue) at stage 4 of cytokinesis. (D-G'') HeLa cells at stages 1 (D-D''), 2 (E-E'',F-F'') and 4 (G-G'') of cytokinesis were triple-stained with anti-AMSH antibody (D-G), anti-tubulin antibody (D'-G', red) and TO-PRO-3 (D'-G', blue). B''-G'' are merged images. Asterisks in E,E'',F,F'' indicate the midbody remnants in neighboring interphase cells. Insets show high-magnification images of the central spindle/midbody regions. Scale bars: 10 µm.





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