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Figure 2


Fig. 2. Ubiquitylated proteins localize to the central spindle during cytokinesis. (A-D'') HeLa cells at stages 1 (A-A''), 2 (B-B''), 3 (C-C'') and 4 (D-D'') of cytokinesis were triple-stained with FK2 (A-D), anti-tubulin antibody (A'-D', red) and TO-PRO-3 (A'-D', blue). (E-F'') HeLa cells were transfected with HA-tagged Ub, and triple-stained with anti-HA antibody (E,F), FK2 (E', green) or anti-AMSH antibody (F', green) and TO-PRO-3 (E',F', blue). A''-F'' are merged images. Insets show high-magnification images of the central spindle/midbody regions. Scale bars: 10 µm.





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